Ruffell Lab

Research Articles

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Hänggi et al. • STAR Protocols 2025

Protocol for specific induction of apoptosis or necroptosis in murine tumors

Hänggi et al. • Cancer Cell 2024

Interleukin-1a release during necrotic-like cell death generates myeloid-driven immunosuppression that restricts anti-tumor immunity

Celias et al. • STAR Protocols 2022

Detection of exogenous DNA uptake by murine dendritic cells

Gardner et al. • JITC 2022

TIM-3 blockade enhances IL-12-dependent anti-tumor immunity by promoting CD8⁺ T cell and XCR1⁺ dendritic cell spatial co-localization

El-Kenawi et al. • Cancer Research 2021

Macrophage-derived cholesterol contributes to therapeutic resistance in prostate cancer

de Mingo Pulido et al. • Immunity 2021

The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake

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de Mingo Pulido et al. • Cancer Cell 2018

TIM-3 regulates CD103⁺ dendritic cell function and response to chemotherapy in breast cancer

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Review Articles

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Hänggi & Ruffell • Trends in Cancer 2023

Cell death, therapeutics and the immune response in cancer

Li, DeNicola & Ruffell • IRCMB 2022

Metabolism in tumor-associated macrophages

Gardner, de Mingo Pulido & Ruffell • Frontiers in Immunology 2020

Dendritic Cells and their Role in Immunotherapy

El-Kenawi, Hänggi & Ruffell • CSH Perspectives 2020

The Immune Microenvironment and Cancer Metastasis

DeNardo & Ruffell • Nature Reviews Immunology 2019

Macrophages as Regulators of Immunity and Immunotherapy

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Gardner & Ruffell • Trends in Immunology 2016

Dendritic Cells and Cancer Immunity

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de Mingo Pulido & Ruffell • Adv in Cancer Research 2015

Immune Regulation of the Metastatic Process: Implications for Therapy

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Ruffell & Coussens • Cancer Cell 2015

Macrophages and Therapeutic Resistance in Cancer

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Preview Articles

Li & Ruffell • Immunity 2022

Cytokines drive prostate cancer lineage plasticity

Rodriguez & Ruffell • Cancer Cell 2021

Cavity macrophages stop anti-tumor T cells

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Hänggi & Ruffell • Cancer Cell 2019

Oncogenic KRAS Drives Immune Suppression in Colorectal Cancer

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Gardner & Ruffell • Immunity 2017

moDCs, Less Problems

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El-Kenawi & Ruffell • Cancer Cell 2017

Inflammation, ROS, and Mutagenesis

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Gardner & Ruffell • Nature Cell Biology 2017

Reducing Interferon'ce in Stem Cells

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Pilon-Thomas & Ruffell • Cancer Cell 2016

Tipping the Balancing ACT

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Collaborations

Phadke MS, Li J, Sriramareddy S, Rodriguez PC, Ruffell B, Luca VC, Tran TTT, Chen YA, Smalley KSM. (2025). Identification of anti-TIM-3 based checkpoint inhibitor combinations with activity in immunotherapy refractory melanoma models. J. Immunother. Cancer 13(8): e012011. PMID 40825611

Senent Y, Remírez A, Repáraz D, Llopiz D, Celias DP, Sainz C, Entrialgo-Cadierno R, Auarez L, Rouzaut A, Alignani D. Tavira B, Lambris JD, Woodruff TM, de Andrea CE, Ruffell B, Sarobe P, Ajona D, Pio R (2025). The C5a/C5R1 axis promotes migration of tolerogenic dendritic cells to lymph nodes, impairing the anticancer immune response. Cancer Immunol. Res. 13(3): 384-399. PMID 39666368

Kloosterman DJ, Erbani J, Boon M, Farber M, Handgraaf SM, Ando-Kuri M, Sánchez-López E, Fontein B, Mertz M, Nieuwland M, Liu NQ, Forn-Cuni G, van der Wel NN, Grootemaat AE, Reinalda L, van Kastren SI, de Wit E, Ruffell B, Snaar-Jagalska E, Petrecca K, Brandsma D, Kros A, Giera M, Akkari L. (2024) Macrophage-mediated myeloid recycling fuels brain cancer malignancy. Cell 187(19): 5336-5356. PMID 39137777

Ozakinci H, Song X, Nazario GS, Lila T, Chen B, Simpson T, Nguyen JV, Segura CMM, Thompson ZJ, Thapa R, Rose TA, Haura EB, Pellini B, Yu X, Ruffell B, Chen TS, Boyle TA, Beg AA. (2024) Rapid autopsy to define dendritic cell spatial distribution and T cell association in lung adenocarcinoma. J. Immunol. 213(7): 1033-1041. PMID 39120462
Lockhart JH, Ackerman HD, Lee K, Adbalah M, Davis AJ, Hackel N, Boyle TA, Saller J, Keske A, Hanggi K, Ruffell B, Stringfield O, Cress WD, Tan AC, Flores ER. (2023) Grading of lung adenocarcinoma with simultaneous segmentation by artificial intelligence (GLASS-AI). npj Precision Oncology 7(1): 68. PMCID: PMC10354042
Zheng H, Xiaoqing Y, Ibrahim ML, Foresman D, Xie M, Johnson JO, Boyle TA, Ruffell B, Perez BA, Antonia SJ, Ready N, Saltos AN, Cantwell MJ, Beg AA. (2023) Combination IFNB and membrane-stable CD40L maximize tumor dendritic cell activation and lymph node trafficking to elicit systemic T-cell immunity. Cancer Immunol Res 11(4): 466-458. PMID36757308

Lester DK, Burton C, Gardner A, Innamarato P, Kodumudi K, Liu Q, Adhikari E, Ming Q, Williamson DB, Frederic DT, Sharova T, White MG, Markowitz J, Cao B, Nguyen J, Johnson J, Beatty M, Mockabee-Macias A, Mercurio M, Watson G, Chen P-L, McCarthy S, MoranSegura C, Messina J, Thomas KL, Darville L, Izumi V, Koomen JM, Pilon-Thomas SA, Ruffell B, Luca VC, Haltiwanger RS, Wang X, Wargo JA, Boland GM, Lau EK. (2023) Fucosylation of HLA-DRB1 regulates CD4+ T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacy. Nature Cancer 4(2): 222-239. PMID 36690875

Mandula JK, Chang S, Mohamed E, Jimenez R, Sierra-Mondragon RA, Chang DC, Obermayer AN, Moran-Segura CM, Das S, Vazquez-Martinez JA, Prieto K, Chen A, Smalley KSM, Czerniecki B, Forsyth P, Koya RC, Ruffell B, Cubillos-Ruiz JR, Munn DH, Shaw TI, Conejo-Garcia JR, Rodriguez PC. (2022) Ablation of the endoplasmic reticulum stress kinase PERK induces paraptosis and type I interferon to promote anti-tumor T cell responses. Cancer Cell 40(10): 1145-1160. PMID 36150390

Ming Q, Celias DP, Wu C, Cole AR, Singh S, Mason C, Dong S, Tran TH, Amarasinghe GK, Ruffell B, Luca VC (2022). LAG3 ectodomain structure reveals functional interfaces for ligand and antibody recognition. Nature Immunology 23(7): 1031-1041. PMID 35761082

Maller O, Drain AP, Barrett AS, Borgquist S, Ruffell B, Zakharevich I, Thanh PT, Gruosso T, Kuasne H, Lakins JN, Acerbi I, Barnes JM, Nemkov T, Chauhan A, Gruenberg J, Nasir A, Bjarnadottir O, Werb Z, Kabos P, Chen YY, Hwang SE, Park M, Coussens LM, Nelson AC, Hansen KC, Weaver VM. (2020). Tumor-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression. Nature Materials.

Mohamed E, Sierra RA, Trillo-Tinoco J, Cao Y, Innamarato P, Payne KK, de Mingo Pulido A, Mandula J, Zhang S, Thevenot P, Biswas S, Abdalla SK, Costich TL, Hänggi K, Anadon CM, Flores ER, Haura E, Mehrotra S, Pilon-Thomas S, Ruffell B, Munn D, Cubillos-Ruiz JR, Conejo-Garcia JR, & Rodriguez PC (2020) ER stress sensor PERK governs myeloid cell-driven immunosuppression in tumors through NRF2-dependent inhibition of STING signaling. Immunity 52(4): 668-682.

Trillo-Tinoco J, Sierra RA, Mohamed E, Cao Y, de Mingo-Pulido A, Gilvary D, Anadon CM, Costich TL, Wei S, Flores E, Ruffell B, Conejo-Garcia JR, & Rodiquez PC (2019) AMPKa1 intrinsically regulates the function and differentiation of tumor-related MDSC. Cancer Research.

El-Kenawi A, Gatenbee C, Robertson-Tessi M, Bravo R, Dhillon J, Balagurunathan Y, Berglund A, Visvakarma N, Ibrahim-Hashim A, Choi J, Luddy K, Gatenby R, Pilon-Thomas S, Anderson A, Ruffell B, Gillies R (2019) Acidity promotes tumor progression by altering macrophage phenotype in prostate cancer. Br. J. Cancer 121(7): 556-566.

Reddy JP, Atkinson RL, Larson R, Burks JK, Smith D, Debeb BG, Ruffell B, Creighton C, Bambhroliya A, Reuben J, Van Laere SJ, Krishnamurthy S, Symmans WF, Brewster A, Woodword WA (2018). Mammary stem cell and macrophage markers are enriched in normal tissue adjacent to inflammatory breast cancer. Breast Cancer Res. Treat. 171 (2): 283-293.

Betts CB, Pennock ND, Ruffell B, Schedin PJ. (2018) Mucosal immunity in the female murine mammary gland. J. Immunol. 201: 734-746.

Bergstrom CP, Ruffell B, Ho CMT, Higano CS, Garzotto M, Beer TM, Graff JN. (2017) Docetaxel and mitoxantrone prior to radical prostatectomy in men with high risk prostate cancer: Ten-year follow up and immune correlates. Anti-Cancer Drugs 28 (1): 120-126.

Wolfe AR, Trenton NJ, Debeb BG, Larson R, Ruffell B, Chu K, Hittelman W, Diehl M, Reuben JM, Ueno NT, Woodward WA. (2016) Mesenchymal stem cells and macrophages interact through IL-6 to promote inflammatory breast cancer in pre-clinical models. Oncotarget 7 (50): 82482-82492.

Zheng H, Zhao W, Bronk C, Massengill M, Yan C, Zie M, Noyes D, Yoder S, Massengill C, Martinez GV, Afzal R, Chen Z, Haura EB, Antonia SJ, Ruffell B, Beg AA. (2016) HDAC inhibitors enhance tumor immunogenicity and T cell infiltration and function to augment response to PD-1 immunotherapy. Clin. Cancer Res. 22 (16): 4119-4132.

Juric V, Ruffell B, Evason KJ, Che L, Hu J, Chen X, Bishop JM. (2015) Monocytes promote liver carcinogenesis in an oncogene-specific manner. J Hepatol. 64 (4): 881-890.

Wolfe AR, Debeb BG, Lacerda L, Larson R, Bambhroliya A, Huang X, Bertucci F, Finetti P, Birnbaum D, Van Laere S, Diagaradjan P, Ruffell B, Trenton NJ, Chu K, Hittelman W, Diehl M, Levental I, Ueno NT, Woodward WA. (2015) Simvastatin prevents triple-negative breast cancer metastasis in pre-clinical models through regulation of FOXO3a. Breast Cancer Res Treat 154(3): 495-508.

Full list of Dr. Ruffell's publications

NCBI MyBibliography • Google Scholar • Scopus Database • ORCID Database