Protocols

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Cell and Tissue Analysis

Flow Cytometry - Human

Flow Cytometry - Mouse

Immunohistochemistry

Immunofluorescence

In Vivo & Ex Vivo Studies

Freezing cells for FACS or FC

Mammary tumor implantation

Isolation of DCs from spleens

Bone marrow DC generation

Bone marrow chimeras

 

Molecular Biology

CRISPR/Cas9 Cloning

Genotyping

 

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Cellular Assays

DNA uptake assay using DCs 

Induction of cell death

 

Available Resources

Plasmids on Addgene

Code on Github

Datasets on GEO

1) scRNAseq of CD45+ cells in PyMT-B6 tumors