Laboratory:

The lab is situated on the second floor of the Stabile Research Building (SRB) which was completed in 2004. The lab set up is “open” which means that several labs share an open floor plan. This has the advantage of promoting interdisciplinary research and collaboration while making the best use of space. The Lynch Lab is comprised of two bays (Approx. 1000 sq ft)  for up to 8 personnel.  All of the labs on the second floor have bay windows that give lots of natural light and a sense of space. The Lynch Lab contains all the necessary equipment to perform molecular and cell biology experiments. The lab contains, two BSL2 cabinets for tissue culture, CO2 incubators, LN2 cell bank, a fume hood, tissue histology and processing equipment, a shared walk-in fridge, centrifuges and apparatus for DNA and protein analysis (electrophoresis and transfer). The open lab design ensures shared access to spectrophotometers, gel documentation systems, LI-COR Odyssey for western blot analysis and orbital incubators. The mouse vivarium on the Moffitt campus is state of the art and is run by dedicated veterinarians and animal care technicians of the USF Department of Comparative Medicine.

Equipment:

2x BSL biosaftey cabinets
2x 5% CO2 Incubators
1x Gravity Convection Incubator
1x Inverted microscope
1x Dissecting Microscope
1x Benchtop Centrifuge
4x Eppendorf microfuges
2x Eppendorf refrigerated microfuges
1x ABI Biosystems Veriti PCR Machine
2x Freezers (-20C) and Fridges (4C)
1x Freezer (-80 C)
1x LN2 Cell Cryo Tank
2x Digital heat blocks
3x Nutators
1x High Power Microwave

Lab image

Lab image

Computer and Peripherals:

All personnel are provided with Dell or Mac desktops or laptops depending on their individual requirements. Printers (color and B&W) are on a shared drives. All software (Microsoft, GraphPad, Adobe Photoshop etc.) is provided. For software requiring extensive processing capabilities, such as Metamorph, the shared resource labs highlighted below (microarray/microscopy) provide designated computers for data and image analysis.

Shared Resources:

Molecular Genomics ; DNA/RNA analysis/sequencing, qPCR and shRNA and microarray.
Microscopy ; Confocal/fluorescent/widefield microscopy, laser capture, slide scanning.
Flow Cytometry ; FACS (Scan/Calibur/Vantage/Aria) Luminex bead array and MACs separator
Proteomics ; 2D-DIGE, LC-MS, MALDI-TOF
Small Animal and Mouse Imaging ; IVIS, ART, MRI, Ultrasound, PET/CT/SPECT
Biostatistics ; Statistical support in Experimental design, approach and interpretation
Cancer Informatics ; Support in interrogating cancer registry data
Chemical Biology ; Design of chemical probes and compounds
Tissue Core ; Processing of tissues and access to Moffitt cancer tissue banks