Welcome to the Mahajan Lab Website

Research Overview

A major interest in our lab is to determine how tyrosine phosphorylation regulates epigenetic processes that affect cellular homeostasis and cancer cell survival. We employ multiple tyrosine kinases to investigate these processes. The examples are:

ACK1/TNK2 : An oncogene with novel epigenentic activity  in prostate & breast cancers

WEE1 : A novel epigenetic modulator & its significance in various malignancies

ABL1 : A proto-oncogene with nuclear tyrosine kinase activity

Some of the major findings of our lab include:

  • We demonstrated for the first time that WEE1 is an epigenetic kinase phosphorylating histone H2B at Tyrosine 37, regulating global histone output
  • Recently, we have identified a novel WEE1 epigenetic inhibitor
  • We demonstrated that ACK1 (also known as TNK2) kinase plays crucial role in drug-resistance of prostate, breast & lung cancers
  • We identified a novel AKT phosphorylation site, Tyrosine 176 that correlated with prostate, breast and pancreatic cancer progression to metastatic stages
  • Androgen receptor (AR) was shown to be phosphorylated by ACK1 at Tyrosine 267 and 363 in castration resistant prostate cancer or CRPC
  • ACK1 phosphorylates histone demethylase KDM3A (JHDM2A) at Tyrosine 1114 to regulate HOXA1, facilitating tamoxifen-resistance of the breast cancer
  • We have developed a new class of ACK1 small molecule inhibitor

Mahajan K et al. J. Cell. Physiol. 224: 327-333, 2010.

 

Current Projects

    • ACK1 epigenetic signaling
    • ACK1/TNK2 knockout mice
    • Characterization of novel class of ACK1 inhibitors
    • WEE1 epigenetic signaling
    • Characterization of novel class of WEE1 small molecule inhibitors
    • Role of ACK1-AR signaling in triple negative breast cancer
    • Long non-coding RNAs as chromatin modifiers

Postdoctoral Opportunities

We are in recruitment drive and will hire up to 3 new postdocs. Send your CV to

Nupam.mahajan@moffitt.org

Nupam Mahajan, PhD
Associate Professor
Moffitt Cancer Center

Contact:
SRB-2, Office # 22043
Moffitt Cancer Center
12902 Magnolia Drive
Tampa, Florida 33612

Email:
Nupam.mahajan@moffitt.org

Phone:
Office: 813-745-6684
Lab: 813-745-4078
Fax:: 813-745-6748

Funding Sources: